Krüger lab - CECAD
REVERSIBLE TRANSLOCATION OF GLUT4 VESICLES BY INSULIN-DEPENDENT E3-LIGASES
Insulin secretion induces the activation of the insulin-signaling pathway resulting in a translocation of GLUT4 vesicles to the plasma membrane facilitating glucose influx into the cell. The project will be focused on the characterization of insulin-dependent activation of E3 ligases and will decipher how protein ubiquitylation modulates the localization of signaling molecules and cellular vesicles. The project will use cutting-edge mass spectrometry and bioinformatics to show that reversible PTMs and transient protein-protein interactions are associated with changes of the GLUT4 localization after insulin stimulation.
Luisa Schmidt
PhD student since April 2020
EMail: luisa.schmidt[at]uni-koeln.de
"I am Luisa and I studied biochemistry at the Julius-Maximilians-University of Würzburg. Proteomic data analysis is a central aspect of my PhD which aims to characterise the spatial proteome of muscle-junction-tendon. This requires sophisticated method development including sample preparation, LC-MS/MS setups, as well as the downstream bioinformatics."
Theresa Bock
Graduated in November 2023
EMail: tbock2[at]uni-koeln.de
"My name is Theresa and I studied Biology and Biochemistry in Cologne. In my master’s program, I focused my studies on protein biochemistry which eventually broad me to the Krüger lab where I am working on a mitochondrial protein in cardiac and skeletal muscle, predominantly by using mass spectrometry-based approaches."
Publications
- FGF21 modulates mitochondrial stress response in cardiomyocytes only under mild mitochondrial dysfunction. Croon M, Szczepanowska K, Popovic M, Lienkamp C, Senft K, Brandscheid CP, Bock T, Gnatzy-Feik L, Ashurov A, Acton RJ, Kaul H, Pujol C, Rosenkranz S, Krüger M, Trifunovic A Sci Adv. 2022 Apr 8;8(14):eabn7105. doi: 10.1126/sciadv.abn7105. Epub 2022 Apr 6. PMID: 35385313
- Low kindlin-3 levels in osteoclasts of kindlin-3 hypomorphic mice result in osteopetrosis due to leaky sealing zones. Klapproth S, Richter K, Türk C, Bock T, Bromberger T, Dominik J, Huck K, Pfaller K, Hess MW, Reichel CA, Krüger M, Nakchbandi IA, Moser M. J Cell Sci. 2021 Oct 27:jcs.259040. doi: 10.1242/jcs.259040. Online ahead of print. PMID: 34704600
- PERM1 interacts with the MICOS-MIB complex to connect the mitochondria and sarcolemma via ankyrin B. Bock T, Türk C, Aravamudhan S, Keufgens L, Bloch W, Rozsivalova DH, Romanello V, Nogara L, Blaauw B, Trifunovic A, Braun T, Krüger M. Nat Commun. 2021 Aug 12;12(1):4900. doi: 10.1038/s41467-021-25185-3. PMID: 34385433
- Phosphoproteomics of the developing heart identifies PERM1 - An outer mitochondrial membrane protein. Aravamudhan S, Türk C, Bock T, Keufgens L, Nolte H, Lang F, Krishnan RK, König T, Hammerschmidt P, Schindler N, Brodesser S, Rozsivalova DH, Rugarli E, Trifunovic A, Brüning J, Langer T, Braun T, Krüger M. J Mol Cell Cardiol. 2021 May;154:41-59. doi: 10.1016/j.yjmcc.2021.01.010. Epub 2021 Feb 5. PMID: 33549681
- Exercise-dependent increases in protein synthesis are accompanied by chromatin modifications and increased MRTF-SRF signalling. Solagna F, Nogara L, Dyar KA, Greulich F, Mir AA, Türk C, Bock T, Geremia A, Baraldo M, Sartori R, Farup J, Uhlenhaut H, Vissing K, Krüger M, Blaauw B. Acta Physiol (Oxf). 2020 Sep;230(1):e13496. doi: 10.1111/apha.13496. Epub 2020 May 30. PMID: 32408395
- High-throughput proteomics fiber typing (ProFiT) for comprehensive characterization of single skeletal muscle fibers. Kallabis S, Abraham L, Müller S, Dzialas V, Türk C, Wiederstein JL, Bock T, Nolte H, Nogara L, Blaauw B, Braun T, Krüger M. Skelet Muscle. 2020 Mar 23;10(1):7. doi: 10.1186/s13395-020-00226-5. PMID: 32293536