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PTMs OF ENDOCYTIC ADAPTORS MEDIATE THEIR REROUTING FROM PLASMA MEMBRANE TO AUTOPHAGOSOMES

In neurons, the formation of autophagosomes and their turnover are spatially segregated, since distally-formed autophagosomes require the microtubule-based transport to the cell soma, where the majority of lysosomes is located. AV transport is promoted by the endocytic adaptor AP-2, which needs to relocalize from the plasma membrane, where it orchestrates the clathrin-mediated endocytosis, to distally-formed autophagosomes. However, how AP-2 switches between its function in clathrin-mediated endocytosis and autophagy is currently unknown. This project focus on precise mechanisms which regulate the cellular relocalization of AP-2 in neurons.

Selected Publications

  • Kononenko, N.L., G.A. Classen, M. Kuijpers, D. Puchkov, T. Maritzen, A. Tempes, A.R. Malik, A. Skalecka, S. Bera, J. Jaworski, and V. Haucke (2017), Retrograde transport of TrkB-containing autophagosomes via the adaptor AP-2 mediates neuronal complexity and prevents neurodegeneration. Nat Commun. 8: p. 14819.

  • Kononenko N.L (2017), Lysosomes convene to keep the synapse clean. J Cell Biol. 216:2251.

  • Kononenko N.L. & Haucke V (2015), Molecular mechanisms of presynaptic membrane retrieval and synaptic vesicle reformation. Neuron. 85, 484-496.